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SAS institute
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Verlag GmbH
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Image Search Results
Journal: Cellular and Molecular Gastroenterology and Hepatology
Article Title: Myeloid Deletion of Cdc42 Protects Liver From Hepatic Ischemia-Reperfusion Injury via Inhibiting Macrophage-Mediated Inflammation in Mice
doi: 10.1016/j.jcmgh.2024.01.023
Figure Lengend Snippet: Myeloid deletion of Cdc42 enhances the M2-type differentiation of macrophages via regulating STATs signaling. THP1 cells were pretreated with PMA (100 ng/mL) and ML141 (10 μM) for differentiation and then stimulated with LPS (100 ng/mL) and IFN-γ (20 ng/mL) for 1 hour for M1 induction, or IL4 and IL13 (both at 40 ng/mL) for 48 hours for M2 induction. The images ( A ) and quantitative results ( C–F ) of the phosphorylation of STAT1, STAT3, and STAT6 and expressions of SOCS3 were detected by Western blot analysis in THP1 cells, n = 3. The images ( B ) and quantitative results ( G–J ) of the phosphorylation of STAT1, STAT3, and STAT6 and expressions of SOCS3 were measured by Western blot analysis in BMDMs stimulated with LPS (100 ng/mL) for 3 hours or IL4 (40 ng/mL) for 48 hours, respectively, n = 3. n. s., no significance; ∗ P < .05; ∗∗∗ P < .001.
Article Snippet: The proteins were extracted and resolved by sodium dodecyl sulfate–polyacrylamide gel electrophoresis and transferred to a nitrocellulose membrane (GE Healthcare), which will be blocked with 5% BSA or skimmed milk in Tris-buffered saline containing 0.1% Tween 20 and probed with antibodies against iNOS (Proteintech, 22226-1-AP), ARG1 (Proteintech, 66129-1-AP), IL10 (proteintech,60269-1-Ig), IL6 (
Techniques: Western Blot